Low target consumption is a benefit to Optimer discovery, preventing excessive consumption of rare or high cost targets.
For small molecule targets, we require 25 µmoles of target molecule, 10 µmoles of any counter-targets and 10 mL of matrix for the entire Optimer discovery process.
For protein targets, we require 70 nmoles of target, 30 nmoles of any counter-targets and 10 mL of matrix for the entire Optimer discovery process.
For cell targets, we require 2-3 aliquots of 1×106 target cells and 2-3 aliquots of 1×106 counter-target cells if the cells are being cultured in-house. We require 30 aliquots of 1×106 target cells and counter-target cells if not being cultured in-house. We require 10 mL of matrix for the entire Optimer discovery process.
If you require assistance in sourcing your target molecule we can support you with finding suppliers or target costs can be incorporated into your Optimer discovery project.
The high specificity targeting of Optimer binders to functional epitopes means that the discovery process is still effective when targeted to protein subunits (where the whole protein cannot be obtained) or tag-stabilised proteins (for proteins that are unstable in vitro), with final Optimer products showing no cross-reactivity and binding to full length functional proteins.