Low target consumption is a benefit of Optimer® discovery, preventing excessive consumption of rare or high cost targets.
For small molecule targets, we require 26 µmoles of target molecule, 11 µmoles of any counter-targets and 10 mL of matrix for the entire Optimer® discovery process.
For protein targets, we require 70 nmoles of target, 30 nmoles of any counter-targets and 20 mL of matrix for the entire Optimer® discovery process.
For cell targets, we require 2-3 aliquots of 1×106 target cells and 2-3 aliquots of 1×106 counter-target cells if the cells are being cultured in-house. We require 40 aliquots of 1×106 target cells and counter-target cells if not being cultured in-house. We require 10 mL of matrix for the entire Optimer® discovery process.
If you require assistance in sourcing your target, we can support you with finding suppliers or target costs can be incorporated into your Optimer® discovery project.
The high specificity targeting of Optimer® binders to functional epitopes means that the discovery process is still effective when targeted to protein subunits (where the whole protein cannot be obtained) or tag-stabilised proteins (for proteins that are unstable in vitro), with final Optimer® products showing no cross-reactivity and binding to full length functional proteins.