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Customer case studies

Aptamer Group > Optimer binders > Customer case studies

Our custom Optimer expertise tailored for your solution

We work to deliver custom Optimer binders that enable and can scale with your project from conception to commercialization. Explore how we’ve worked with other industry partners to enable optimized solutions to accelerate their pipelines.

Case study 1–Rapid Optimer development to enable custom QC assay

Case study 2–Affinity chromatography binders with specific elution conditions

Rapid development of highly selective Optimers to enable a custom quality control assay

Customer need: Following initial failures with antibody development, the partner required rapid development of an Optimer to discriminate between a citrullinated and native form of the same protein to perform quality control assessments of production.

Aptamer Group Approach: A specifically designed Optimer discovery campaign incorporating strict negative controls during multiple rounds of discovery was developed. This drove selectivity between the two protein forms and ensured specificity for the native protein. Biolayer interferometry shows the specificity of the Optimer for the native form with no cross-reactivity to the citrullinated protein.

Highly selective aptamer

Customer benefit: Initial Optimer binders were offered to the customer for testing after 5 weeks and the final effective Optimer delivered for incorporation into the functional quality control assay in just 10 weeks, allowing the customer to meet project milestones on time, with the required assay specifications.

Development of high-performance affinity chromatography binders with specific elution conditions

Customer need: Customer required affinity chromatography binders to purify a plasma protein with specific mild elution conditions to ensure the protein was not denatured during the process.  

Aptamer Group Approach: The discovery campaign was customized to identify Optimer binders that specifically bound to and eluted the plasma protein target under very restricted buffer conditions. This was done to maintain the activity of the purified protein throughout the process. We also incorporated selection rounds to ensure high target specificity and achieve a high level of product purity from the complex sample matrix. Following discovery, we engineered different batches of the custom Optimer with either amine- or thiol-reactive groups for easy conjugation to different resins for customer validation.

Customer benefit: The customer was able to purify the target protein from plasma samples with the desired level of purity and maintain the protein activity through the use of Optimer binders tailored to mild elution conditions. Simple functionalisation of the Optimer allowed for flexible optimisation of affinity chromatography resins to enable further development.

 

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