Optimer® – Delivering complete control over affinity ligand design, quality and security of supply.
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Understanding of customer needs and project aims. Work schedule developed following established, standardised approach to meet clear milestones via data-driven model.
Specific proprietary high-throughput selection processes for different target classes:
- Cells
- Proteins and peptides
- Small molecules
Adaptable selection system with the ability to: counter-select against related targets, select in end-use buffer and matrix conditions, select in the presence/ absence of ligands, screen mutant vs wild-type, select for specific binding kinetics and performance.
Aptamer pools are assessed for target affinity and the aptamer population is cloned out. Individual clones are isolated from the population for next-generation sequencing to determine the diversity in the aptamer repertoire. Individual aptamers are ranked according to target affinity by biolayer interferometry or other project-defined criteria. Target affinity of the best aptamer clone is increased and cost-effective manufacture ensured via identification of the minimal performing fragment of the aptamer to generate an Optimer.
Optional services from proof-of-concept to a defined and functional assay. Range of assays available in-house, including ELISA, flow cytometry, cell-based inhibition/activation assays, affinity chromatography, WB, SPR, fluorescence microscopy and IHC.
Sequencing, synthesis and quality control processes to ensure delivery of a functional Optimer.
The Optimer® platform delivers next-generation aptamer tools that are enabling researchers and developers across the spectrum of the life sciences.
Find out about Optimer® advantages here and the range of targets we can work with here.
Optimer® binders have been successfully developed for a range of applications, including:
- therapeutic inhibitors and modulators
- drug delivery vehicles
- protein purification and separation
- biomarker screening and validation
- in vitro research
- quality control analysis
- pharmacokinetic and drug assays
- in vitro diagnostic assays
For more information on the Optimer® platform and how we customise the discovery process to provide perfectly tuned aptamers for you, download the platform brochure below.