Specific, stable Optimer reagents for rapid flow cytometry results
Optimers have been successfully developed to a variety of protein and cellular targets and demonstrated excellent performance in flow cytometry applications.
Flow cytometry enables the rapid multiparametric analysis of single cells in a solution. For both flow cytometry and fluorescence-activated cell sorting (FACS) applications, the specificity and stability of the affinity reagent are crucial for reliable results. Optimers deliver high affinity and target specificity tailored according to the specific assay conditions. This is combined with the flexibility to incorporate the full range of fluorescent labels consistently and cost-effectively for flow cytometry panels that meet your needs.
Our proprietary selection methods ensure Optimers recognise the native antigen structure for assured performance in the end-use assay. The nature of the target protein does not need to be known – Optimer selection can be ‘hypothesis-free’ and target discrete cell populations for responsive vs no response, healthy vs disease or populations at different stages of disease. With rapid Optimer selection performed in as little as 4 weeks, working with Optimers can accelerate processes to drive discovery in flow cytometry.
- High signal from high purity of target-specific Optimers
- Reproducible results from standardised and targeted conjugation and batch-to-batch consistency
- Broad range of targets possible with both peptides, proteins and cells
- Target-specific Optimers can be selected and produced in as little as 4 weeks
- Cost-effective synthetic manufacture for security of supply
Optimers were selected against the human multiple myeloma NCI-H929 cell line, with counter-selection against the leukaemia erythromyeloid K-562 cell line to support a hypothesis-free biomarker discovery program with therapeutic partners. Flow cytometric analysis demonstrated clear targeting to the desired cell type allowing simple cell analysis and sorting via FACS.
Flow cytometric analysis of live cells shows clear targeting of the desired cell population. Cells were blocked with for 30 minutes at 4°C using buffers contain 1mg/ml Salmon Sperm DNA and 2% FCS prior to aptamer incubation. Aptamers was incubated with cell lines for 30 minutes at 4°C (35 pmoles per 1×105 cells). The selected Optimer showed no interaction with the negative cell line. Target specific Optimer (green), non-specific aptamer (blue), no aptamer control (red).
Custom Optimers for flow cytometry
Aptamer Group’s established processes can rapidly select Optimers for your target that are compatible with your required flow cytometry target and conditions. Following selection, our in-house expert team can work with you to support the development of your reagents in your specific flow cytometry process and platform, from proof-of-concept through to commercialisation.