Specific and sensitive biosensor ligands in just 4 weeks
Biosensors are analytical devices used to detect the presence or concentration of a biological analyte, such as a biomolecule, a biological structure, or a microorganism. Each biosensor is composed of three elements: a recognition element that binds to the analyte and produces a signal, a signal transducer, and a reader device that convert a biological response into an electrical signal.
Optimers are proven to be compatible with many well-established biosensor techniques for the detection and monitoring of interactions, including surface plasmon resonance (SPR), bio-layer interferometry (BLI), and isothermal titration calorimetry (ITC). As part of these biosensor platforms, Optimers form powerful tools for basic research, diagnostic assays and the analysis and monitoring of drug discovery and development.
- Highly specific biosensors due to specific target binding of Optimers
- Increased biosensor sensitivity due to the small size of Optimers interactions occur closer to the sensor and a higher packing density is achieved on the biosensor surface
- Optimers can be generated to a wide range of targets for novel targeted biosensor assay
- Targeted functionalisation for directed orientation of Optimers on the biosensor for increased signal detection
- Rapid development with Optimers developed in as little as 4 weeks
Using Optimer selection technology, highly selective biosensor assays were developed for SARS-CoV-2. Optimers were selected for the SARS-CoV-2 S1 protein and showed no interaction with the highly homologous coronavirus S1 proteins within a complex human saliva matrix.
Biolayer interferometry showed Optimers specific for SARS-CoV-2 S1 protein were selective for the target in 10% human saliva matrix with no significant interactions with the highly homologous viral targets.
SPR analysis of the small molecule chemotherapeutic, imatinib, showed a concentration-dependent association/dissociation response with the 5’biotinylated imatinib aptamer immobilised on a streptavidin-coated sensor.
BIAcore results show clear target concentration-dependent interactions between the immobilised biotinylated aptamer on a streptavidin-coated chip and the small molecule chemotherapeutic target, imatinib, injected at a flow rate of 30 µL/min to yield a KD=131 ± 11 nM.
Custom Optimers for biosensors
Aptamer Group’s established processes can rapidly select Optimers for your target that are compatible with your required biosensor application. Following selection, our in-house expert team can work with you to support the development of your reagents in your specific biosensor system, from proof-of-concept through to commercialisation.